FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80080
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.13111888111888112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAT305.846504E-446.6666681
GTTCACC450.00428478731.1111131
GAATAGA1357.850758E-925.92592670
CGAATAG1451.6789272E-824.1379369
TATATGT750.001818244723.3333344
TATTATA850.003760494320.5882362
CAATGAC900.005234939519.4444454
GACGAAT1902.9212606E-718.42105167
ACGAATA2005.0044036E-717.50000268
CGACGAA2056.4816595E-717.07317266
CCGACGA2108.34023E-716.66666665
GCCGACG2454.1515614E-614.28571464
TGCCGAC2701.1331094E-512.96296363
CTGCCGA3003.3410615E-511.66666762
GCTGCCG3451.3836185E-410.14492761
CGCTGCC3602.1256659E-49.72222260
ACGCTGC3904.7447774E-48.97435959
GACGCTG4006.1086926E-48.75000158
TGACGCT4400.0015718957.95454657
CTGACGC4700.00300545857.44680956
Produced by FastQC (version 0.11.5)