FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45147
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.15061908875451305No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCTC150.002211171769.9988946
TAAAGTG150.002211171769.998896
TGTTATA150.002211171769.998892
GTCTTCG150.002211171769.9988938
ACCTGGT200.006909457552.499177
TCCTCGG200.006909457552.499172
ATTCAAC200.006909457552.4991755
GGTTCAG200.006909457552.4991731
GCTTCCT305.787849E-446.7177241
AAAGAGT400.002395140734.99944731
CTGTCTC5700.00252482276.754278770
Produced by FastQC (version 0.11.5)