FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48505
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG570.11751365838573342No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGG150.002212043470.014
TAGACCG150.002212043470.04
GACCGTA150.002212043470.06
CTTAAGA200.00691215852.52
TCTCGAA200.00691215852.527
GCCTCTA200.00691215852.528
ACTATGC305.823577E-446.6666648
TACCCTT305.823577E-446.6666645
AATCCTT501.6864939E-435.06
TAATACT657.84936E-426.9230794
ACGCTGC1759.934795E-414.070
GACGCTG1850.001511292813.24324369
TGACGCT2000.00271582612.24999968
CTGACGC2200.00553738811.13636367
Produced by FastQC (version 0.11.5)