FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54004
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.1462854603362714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCC200.00691639352.528
GCAAGGC200.00691639352.546
GGGTCCC200.00691639352.537
CAACCGT200.00691639352.510
TATAGGA200.00691639352.52
CACACGT200.00691639352.517
CTTCACC350.00124517340.03
ACTGTGG350.00124517340.08
CCGCAGT350.00124517340.035
ATCACAC400.002399142435.029
ATATACA400.002399142435.04
TATAGTA500.007150628627.9999982
GTCTTCT500.007150628627.9999981
CAGAGAC500.007150628627.99999857
Produced by FastQC (version 0.11.5)