FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59949
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940.15679994662129476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA200.00692007152.570
ATAGAGT200.00692007152.530
CTAATAC400.002401233735.03
TATTAAC552.9599466E-431.8181842
TAAAGTA450.004276249631.1111094
AGTAAAG450.004276249631.1111093
TAATACT604.928776E-429.1666684
TAGTTGA500.007156786528.00000224
AGTGATG500.007156786528.0000028
AAGGCTT500.007156786528.0000025
TATTATA850.00374959120.5882362
Produced by FastQC (version 0.11.5)