FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70590
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.10341408131463381No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC150.00222033369.967450
AGCTCTT350.001246278840.00972755
CAATAGA500.007156769328.006814
TGTATGG500.00718161627.98696110
TTAACAG500.00718161627.98696115
AGGCTTA808.543794E-526.25638270
AAGGCTT900.005221511719.44917369
GAAGGCT950.007135316718.42553370
CCGACGA1550.007272861413.55168165
TCTGACG3551.8346551E-49.86155270
CATCTGA3551.8346551E-49.86155268
ATCTGAC3652.4269012E-49.591372569
ACACATC4401.610926E-48.75212865
ACATCTG4056.870837E-48.64407667
CACATCT4300.00124496928.14151466
TACACAT4854.6111128E-47.940074464
ATACACA5057.1098906E-47.625616663
TTATACA5900.0036888196.52701161
Produced by FastQC (version 0.11.5)