FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44244
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640.14465238224392007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCACC200.006908116452.49999650
GTCGTAG200.006908116452.49999656
TAGTATA350.001242716640.04
TTAAAAG400.002394438334.99999661
ATTATAG400.002394438334.9999963
TATTATA901.8883716E-423.3333322
TATATAC1055.35324E-420.03
ATATATA1600.00891630213.1249992
Produced by FastQC (version 0.11.5)