FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69042
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1000.14483937313519307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAG150.00222249969.948557
CGCTGGA200.006904918752.5375536
AGGTAGA200.006924776452.4994546
AAGGTTG200.006924776452.4994545
AGGTTGA450.004280917331.1107886
TGCTATC450.004296151531.08824347
GAAGACT500.00716455427.999716
CATTCAA500.00718998827.9794210
Produced by FastQC (version 0.11.5)