FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68469
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1140.16649870744424483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATC150.002215952170.02
CCGAAAC150.002215952170.070
TGCAGTG150.002215952170.05
TGTGCTA200.00692427952.510
CGTTATC200.00692427952.522
AGTGAAC200.00692427952.58
GCGCTAA200.00692427952.534
ATCCAGT400.00240362735.06
CACATAT450.004280484331.11111312
GATATAA450.004280484331.1111131
GTATATA604.9360667E-429.1666661
TTCAAGA500.007163833427.99999856
TGTTATG500.007163833427.9999982
ATATAGA800.002641854821.8751
AAGAATT1107.3793664E-419.0909159
GTCTCTT4450.00174909987.865168667
TCTTATA4100.0074188717.68292770
CTCTTAT4150.0082685687.590361669
TGTCTCT4750.0033182757.368420666
Produced by FastQC (version 0.11.5)