FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52434
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.20025174505092116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA150.00221304170.01
CAAACTA200.006915251352.522
CTCGAAT200.006915251352.523
CGTAACA200.006915251352.551
AGAGGGT200.006915251352.529
TATTCAT450.004271398331.11111348
TACTATG500.00714871828.0000027
AATAATA1250.001732486916.8000012
Produced by FastQC (version 0.11.5)