FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_DGY2071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_DGY2071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48410
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGATT150.002215125169.97520452
CGATCAC150.002215125169.97520410
GCTTCTA200.006893484452.5356824
GTACACA200.006921761752.48140311
TTGCAAC200.006921761752.48140352
GAGTATA350.001246059539.9858321
ACTTTAC350.001246059539.9858321
TATACAG400.002400854634.9876025
TTACTTC450.004253989631.13225431
TTATATA952.7350092E-422.0974332
Produced by FastQC (version 0.11.5)