FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41720
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG700.16778523489932887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGGC150.00220758670.01800527
TTAAGAT200.006898303552.5135044
CATAATC200.006898303552.5135043
TACAAGG200.006898303552.51350465
TCGGTGG200.00693116952.4505413
CTATATG350.001240318940.010294
GTATAAA400.002389834735.0090031
CTATATA500.007123157428.0072021
Produced by FastQC (version 0.11.5)