FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52944
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810.1529918404351768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGT150.002213276569.99905449
GGAGAAG150.002213276569.99905430
TCTTAGA200.006915984752.4992940
TGTAAAG400.002398859234.9995273
ATACTAT500.00714980127.9996226
AGAATTG500.00714980127.99962236
Produced by FastQC (version 0.11.5)