FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49005
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.13468013468013468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCAT200.006908153652.50842726
ACAGCGA200.006908153652.50842754
TATATGC305.8195036E-446.6741611
ATTCCTC450.004265317731.1161088
GTATATA604.8817546E-429.2011761
AAATTAT500.00713858428.00449438
ATATACA800.00262874221.8785114
Produced by FastQC (version 0.11.5)