FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46770
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610.13042548642292068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA150.002211541470.09
AGAGTCC150.002211541470.08
CATAAAC150.002211541470.040
AGCAAGA200.006910600752.50000428
CGTTGGT200.006910600752.50000414
GGCTTAA200.006910600752.5000041
TATAGAA200.006910600752.5000042
TCCTTAC200.006910600752.5000048
ATACATC200.006910600752.5000046
GATATGC404.52719E-543.75000470
CGATATG501.685561E-435.00000469
TTCCTTA400.002395850135.0000047
CCGACGA652.061435E-532.30769365
CGACGAT703.420486E-530.066
CGCAAAA500.007140937728.00000261
GATTAAT500.007140937728.0000021
GACGATA657.8450877E-426.92307767
GCCGACG808.497387E-526.25000264
ACGATAT800.002629433821.87500268
TGCCGAC1003.8563472E-421.00000263
Produced by FastQC (version 0.11.5)