FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52247
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860.16460275231113747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTC150.00221299370.000016
TTTAAGA200.00691510452.563
CGGCTGT200.00691510452.539
TATTATT1400.003690379715.02
TATATAT1550.00725122113.5483872
Produced by FastQC (version 0.11.5)