FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75801
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150.15171303808656877No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC150.002216874270.09
CTGATCT150.002216874270.050
CGTTCCC200.00692713852.55
GAGATGG200.00692713852.562
GTTTATA305.8445265E-446.6666642
ATAGTCC350.001248364140.06
ACTATAC400.002405253735.03
ATAAGAG450.00428336331.1111133
TAAGACA450.00428336331.1111134
CTCTGTT500.007168621828.00000250
TATTATA900.005232420319.4444452
ACTGTCT1350.002912778915.55555568
CTGTCTC5950.00404947866.470588769
TGTCTCT5950.00404947866.470588770
Produced by FastQC (version 0.11.5)