FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76792
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.16277737264298364No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGG200.006927468352.49999643
CCGAGAA200.006927468352.49999623
AACTCGA200.006927468352.49999655
TATACGT200.006927468352.4999965
TTCCTTG500.007169173628.0000027
AGAGATG500.007169173628.00000256
TGAACAG700.001216152825.05
TATACTG851.2935363E-424.7058835
TTACACA750.00181756323.3333344
ATACTGA850.003759099220.5882366
CTCCTTA1107.390559E-419.09090870
GACTCCT1159.963983E-418.2608768
CGACTCC1200.001325676917.567
ACTCCTT1400.003711582615.069
ACGACTC1450.00468667714.482758566
TGACGCT4905.4093744E-69.28571470
CTGACGC5151.0166532E-58.83495169
TCTGACG5653.2622316E-58.05309868
ATCTGAC6251.1448846E-47.2867
CATCTGA7004.5961348E-46.566
Produced by FastQC (version 0.11.5)