FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60388
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG910.15069219050142413No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACC207.868001E-570.03
GTGGCGT150.00221467670.030
ACCTGCC200.006920322352.518
CTTGGCG200.006920322352.568
TCTCGGC200.006920322352.546
GCGGCAT200.006920322352.532
CCTTGTG200.006920322352.526
GGTTACA352.0638283E-550.01
TATTAGG350.001246339940.02
CCCTATT450.00427650231.1111091
ACTGTTA450.00427650231.11110925
ATATAGA750.001813076323.3333341
TGACGCT1500.00584965714.00000170
CTGACGC1500.00584965714.00000169
ATACACA2852.6808685E-411.05263170
TATACAC2700.002133294710.3703769
TTATACA3000.0051033259.33333468
Produced by FastQC (version 0.11.5)