FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50994
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGA150.002212687670.02
GTCGTCC150.002212687670.058
CGACGAA200.006914157452.528
ACCTTTA200.006914157452.538
CAGACTT200.006914157452.54
TAATACT552.9537678E-431.818184
CTCAACA450.004270298431.11110912
TATATAA450.004270298431.1111092
ATAGAGC450.004270298431.1111093
TATACTT450.004270298431.1111095
ACCTTTT450.004270298431.11110969
CTTATTA500.007146887527.9999988
ATTTCCG500.007146887527.99999861
TTTTATA657.854856E-426.9230772
ATAATAT808.512176E-526.253
TATAATA950.007118775518.4210512
Produced by FastQC (version 0.11.5)