FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66508
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.1473506946532748No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATAC150.002215673270.068
GATCGAC150.002215673270.038
CTGCTTA200.006923414852.513
CTAAGCC200.006923414852.53
TCAGAAC200.006923414852.522
TTATATA450.00427961531.1111094
AAAGTGG450.00427961531.11110919
TTTGTCT500.00716238428.00000264
TATGGAG500.00716238428.00000248
Produced by FastQC (version 0.11.5)