FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP3-A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP3-A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64488
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAAG150.00221536270.015
CTATAGC200.006922449552.52
CCCACAA200.006922449552.540
CATATAC500.00716076828.0000023
GCCGACG808.546725E-526.2570
TGCCGAC1003.8783526E-421.00000269
CTGCCGA1107.3729496E-419.0909168
GCTGCCG1250.001738354816.867
CTTATAC5906.350474E-78.89830570
TCTTATA6151.1547309E-68.53658569
CTCTTAT6351.8277042E-68.26771768
TCTCTTA6956.6138073E-67.55395767
GTCTCTT7301.3235067E-57.191781566
TTATACA5300.0095942416.603773670
TGTCTCT8055.192026E-56.521739565
CTGTCTC8451.0150578E-46.21301864
Produced by FastQC (version 0.11.5)