FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP2-F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP2-F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54123
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG870.16074496979103153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG150.00221482269.9889145
TCCTAGC200.006895502652.540242
GGGAAGA200.006895502652.5402429
TATGCCA200.00692079752.4916868
GATATGT200.00692079752.4916820
TACACCA200.00692079752.491685
TCCTGAA350.001240500740.030662
ATACAAG400.002390161435.0268253
GTATTAT400.00240104734.99445350
GCATTGT400.00240104734.9944538
TATACAG450.004275930631.106185
ATGCAAG500.007123990528.02146128
Produced by FastQC (version 0.11.5)