FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP2-E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP2-E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59919
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.11348654016255279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA150.00221459570.0000115
GAGTCCG150.00221459570.000019
GGTGTCC200.00692007152.58
AGAGTCC200.00692007152.58
TAGGCCT200.00692007152.512
CGTCACT200.00692007152.520
GTACGAC200.00692007152.551
GTATTAT654.7680987E-737.6923071
CTATTAA400.002401233735.01
GTATATG552.9599466E-431.8181841
GTTCAAA800.002638030621.8751
CTGCCGA850.00374959120.58823670
GCTGCCG950.007133077418.42105169
TATTATA950.007133077418.4210512
Produced by FastQC (version 0.11.5)