FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP2-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP2-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48508
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.1298754844561722No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACTG200.00689722552.52863770
AAGGCAG200.00689722552.52863759
GGTGTTA200.00692545952.4744838
GTTTCAC200.00692545952.47448311
TAGAACA200.00692545952.4744834
TGTGCTC200.00692545952.4744838
AACCTGC200.00692545952.47448349
ATCCATT400.002390307835.01909329
AGTTTCA450.004278452631.09599110
ATAGAAT450.004278452631.0959913
TGACGCT1257.598904E-519.61069369
GACGCTG1301.02700404E-418.85643470
CTGACGC1351.3716728E-418.15804968
TCTGACG1704.228297E-516.47957467
CATCTGA2009.526764E-615.75859165
ATCTGAC2009.526764E-615.75859166
ACATCTG2150.00464607411.40156564
Produced by FastQC (version 0.11.5)