FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP2-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP2-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79451
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.14726057570074638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT150.002217268870.044
CGGAATG200.00692836252.528
GAATGTA200.00692836252.59
CTCAACT305.846239E-446.6666644
AAGATCG350.001248727640.00000421
CTTCAGT400.002405949935.01
ATTACTG450.004284595631.1111135
ACCCTGA500.00717067327.9999986
TACTATA500.00717067327.9999982
TATATTA750.001818124323.3333342
TATATAC850.003760249120.5882363
ATATTAT1301.0419719E-418.8461531
Produced by FastQC (version 0.11.5)