FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP1-F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP1-F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92874
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.1367444064000689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGAG252.379569E-455.9999962
AATACGT252.379569E-455.9999962
TGTGCAC200.00693202752.50000446
CTCCTCT350.001249817740.09
GAGAGTT400.00240803635.00000422
ATAACAC400.00240803635.0000043
CTTATTC450.00428828931.1111133
GATATAC450.00428828931.1111131
GACATAG500.007176818327.9999981
TAAACAC657.9040683E-426.9230775
TATATTG1003.8962017E-421.05
TATTAAA1200.001328526917.52
TATTATT2102.6110816E-413.3333332
TAAAGAA1600.0090139313.1250014
ATATATA3207.890624E-49.8437511
TATATAT3000.0051472439.3333333
Produced by FastQC (version 0.11.5)