FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP1-E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP1-E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107645
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTC150.00221941370.017
CGACATC200.006935010752.517
AAGGCGT200.006935010752.56
CGAGGAA305.855546E-446.66666436
CATATAC450.00429129531.1111093
TAAACAG500.00718181328.0000025
CGAAGAG1003.9017646E-421.00000269
TTTAATA900.005246455319.4444432
ATTTATA1452.4135956E-416.8965531
GACGAAG1250.001748622316.867
TTGAACA1350.002923449715.5555564
ACGAAGA1400.00372455215.068
TATCTTC1450.00470298114.4827595
TATACAC4054.765243E-711.23456870
CTTATAC4351.4453063E-48.85057568
TTATACA4401.6363068E-48.75000169
TCTTATA4652.9757328E-48.27957167
CTCTTAT4703.339485E-48.19148966
GTCTCTT4955.8278505E-47.77777764
TCTCTTA5209.863675E-47.40384665
Produced by FastQC (version 0.11.5)