FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP1-C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP1-C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107676
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1450.1346632490062781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT207.896329E-569.999548
ACTATGC350.001250747239.9997338
CTATAAG450.00428168931.1253621
TTGTGCA500.007182060727.9998159
TTTGTGC500.007182060727.9998158
CATATAC750.001822380923.333183
ATATACT1154.0753035E-521.3042074
TATAAGG1154.0753035E-521.3042072
GTATTAT900.005232583719.4533521
TCATATA1000.0096354917.4998842
TATTATA1250.001748706416.7998892
CTGCCGA1550.007317831713.54829770
ATATAAT2000.002743853712.2556111
Produced by FastQC (version 0.11.5)