FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP1-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP1-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119222
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1410.1182667628457835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAACC200.00693403552.5053540
GTTTGTC350.00125320339.98729711
GTATGGA459.1468406E-538.8928531
TATACCG556.6441735E-638.185715
ACTGTGT400.002409565335.0035678
ACCACCC400.002409565335.0035678
GCCAAGT400.002414533234.98888443
AGGAGTT450.004290987331.11428338
ATACAGA450.004290987331.1142836
CCAATCC500.007181291528.00285340
CGTTTGT500.007196026427.99110810
CAGTACC700.001219387225.0025464
TATAACA952.759289E-422.1075152
TATTGCA900.005246608519.4464262
ACGAAGA1651.5673086E-619.09285470
GTCATAT950.00716945918.422931
ATAACAC950.00716945918.422933
ATACAAT950.00716945918.422936
CGACGAA1752.751085E-618.00183368
ATATACC1000.00963534217.5017834
Produced by FastQC (version 0.11.5)