FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_AP1-A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_AP1-A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12641
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.11075073174590619No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTCG200.006794322752.57
AAGAGGA200.006794322752.570
CGAAGAG200.006794322752.568
TGCCGAC808.0221056E-526.2570
CTGCCGA1003.6442038E-421.069
CGCTGCC1159.3476835E-418.26086867
GCTGCCG1159.3476835E-418.26086868
ACGCTGC1250.001635577316.866
GACGCTG1500.005516431314.065
TGACGCT1600.00845965413.12564
Produced by FastQC (version 0.11.5)