FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20521549
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880284542.895616700279305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAC700.001222914225.0152171
TAGGGGG1901.3578756E-820.2635843
GATCTCG3300.020.14923937
CCGAGAC3650.019.17638229
ACCGATC3500.018.997934
CGATCTC3600.018.46986436
CCCTGGG1150.00100886218.260722
CTAATAC4800.017.5003683
AGGGGGG10150.017.2417014
TAGGTAG2651.888111E-917.1700485
GAGGGGG7150.016.6437053
CCGCACC2201.3968674E-615.9099365
GTATTAG3803.6379788E-1215.6674261
AGACCGA4500.015.55368432
AGTGTGC1350.002951427115.5535338
TATTAGG4550.015.3844892
CCGATCT4350.015.28529535
CGGGGGG19550.015.21765
TCCGCAC1859.0394715E-515.1364564
CTCGTAT5550.015.13368340
Produced by FastQC (version 0.11.5)