FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_bc_anc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_bc_anc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48405
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCATT200.00691207252.57
GTTCGTT200.00691207252.523
GATCTAC200.00691207252.51
AGAGGTA200.00691207252.564
CATCCCT200.00691207252.54
TTGGGAG200.00691207252.531
AGAGACC200.00691207252.54
ATAAGTT450.004268198731.1111134
TCTACAT604.914931E-429.1666663
GTTTGAA500.007143396428.00000236
ATAATAC500.007143396428.0000023
Produced by FastQC (version 0.11.5)