FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_bc_anc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_bc_anc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59382
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT207.8669254E-570.061
ATATGCG252.3725445E-455.99999660
GCGTACT252.3725445E-455.99999652
GAACGGC305.834202E-446.66666822
CATAGTG350.001246172740.028
ATGCGTC350.001246172740.062
GGCATAG350.001246172740.026
GTGCGTG350.001246172740.032
TGCGTAC350.001246172740.051
ACATGAG459.096851E-538.88888513
CTTAAAT400.00240105635.045
GTCTATT400.00240105635.066
CGTCTAT400.00240105635.065
AGTGCGT400.00240105635.031
ATGCGTA400.00240105635.050
TAGTGCG400.00240105635.030
GCGTCTA400.00240105635.064
GAAGAAC652.070801E-532.30769319
TACATGA552.9596218E-431.8181812
TTCTATG450.004275935231.1111092