FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_bc_anc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_bc_anc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77367
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA150.00221704670.06
TCTCGCT150.00221704670.063
CAGAGGC150.00221704670.055
CTCGTTT200.0069276752.50000442
ATAGGTT200.0069276752.50000466
GATCGAA200.0069276752.50000431
GTATTAG450.004283900431.1111131
ACCATCC450.004283900431.1111138
TATTGTA500.007169515428.0000022
TATACAG500.007169515428.0000025
TATTATA952.7490573E-422.1052632
GTATATA1200.001325804217.51
CCGAGCC2200.00558836811.13636467
TGTCTCT7004.5977015E-46.570
CTGTCTC7257.0379005E-46.27586269
Produced by FastQC (version 0.11.5)