FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_Gbc_c82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_Gbc_c82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.10844516741222718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA500.006761127.99999870
CCCACGA901.687558E-423.33333270
AGCCCAC1003.4454165E-421.068
GCCCACG1003.4454165E-421.069
GAGCCCA1106.556524E-419.09090867
CGAGCCC1158.844066E-418.26086866
CCGAGCC1200.001177277117.50000265
TCCGAGC1250.001548188316.864
TCTCCGA1400.003302814215.062
CTCCGAG1400.003302814215.063
ATCTCCG1500.005227803314.061
CATCTCC1600.0080207513.12560
Produced by FastQC (version 0.11.5)