FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_Gbc_c81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_Gbc_c81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78469
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTTG150.002217164370.045
CCTTATA400.002405765235.02
AATACCG501.6960263E-435.05
TCTATCC400.002405765235.03
TAGAAGC450.0042842731.1111094
CCTTTGC450.0042842731.1111091
CAAATAA604.9425836E-429.16666615
TCGTATT500.007170129627.99999863
TTATATT750.001817920823.3333344
ACGAGAC2300.00777900710.65217570
TCCGAGC4850.00408284047.21649570
ACATCTC7301.0457092E-46.712328470
CTCCGAG5300.0096489956.603773669
GTCTCTT13504.3403452E-75.70370470
TGTCTCT13806.6388384E-75.5797169
CACATCT7700.0094303455.454545569
CTGTCTC14151.0751646E-65.44169668
Produced by FastQC (version 0.11.5)