FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_Gbc_c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_Gbc_c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT50.3977724741447892No Hit
ACCCTCACCTAATTCGGCGTTGCGGGGTGGAATTCTAAGCAATTGGGGCG20.15910898965791567No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGAT20.15910898965791567No Hit
AATTAAGACCGTGAAATTAAAACCCATTCCCTTCTGCTGATTTCGTAATA20.15910898965791567No Hit
GAGATGAAGTATCAGGTAATAATTTATACGTATACTACATCGAGACCAAG20.15910898965791567No Hit
GCCTTAGCCGAATGCAATACATCCCTTATAAAATCAATACAATGCGAAAC20.15910898965791567No Hit
CGGTAAGGAATGTGCCGATTTATGGCCAAGAGTTGCATCTAACTCCGGTG20.15910898965791567No Hit
ACGTCGCAGTCCTCAGTCCCAGCTGACAGTATTCCCACAGGCTATAATAC20.15910898965791567No Hit
ATATAAAAGGGCAAAGCTTTTTTTGCTCCCATACTTGCTGAAAATTTTTC20.15910898965791567No Hit
CTTGTAAGTATCACAGTAAAAATATTTTCATACTGTCTATGCAACGAAGG20.15910898965791567No Hit
TAATAGTTCCGGGGCCCGGCCACGGGAGCCGGAACCCCGAAAGGAGAATA20.15910898965791567No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCGGCTGTCTAGATGA20.15910898965791567No Hit
CGTCGAAGATCACTGAACGAATAAAAGATGCGACTGGAACAATAGTCAGT20.15910898965791567No Hit
GGTGAAATGTTGTTATTATGTGTATTCGAAATTTTAGAATTGATCGCCAT20.15910898965791567No Hit
ACATAGAACCGTACACGCTTTTAGCCAAAAAAAAAAAGAAAGGTAGAATA20.15910898965791567No Hit
ATAATGTCCTACGAAAATTGGGGCTACTAGGTGATAAAGAGGACGAAAAA20.15910898965791567No Hit
TCTGTCACCTGCACCACCACAATTGATGATAATCGCCAGAATGACGAATC20.15910898965791567No Hit
TATATAGGCTTACCACAGTTGGATTTAATCAAGCGATATAAAAGCTGGAA20.15910898965791567No Hit
GTTTCTTTGGTGGTAGTGCCCTTAGATTCGGTAACTGGGACAGAAGACTC20.15910898965791567No Hit
GCACTGTTCGCTTCTTCTTCACTCATTAAAAGAGGCTCTATGACGGGTAT20.15910898965791567No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)