FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_Gbc_c73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_Gbc_c73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80080
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.002217408769.99937446
GGACGAA200.006928796452.4995324
AGAGTGG400.002406163434.9996876
GACTAAG1206.366463E-1132.08304670
CCAACTC450.00428497531.11083238
ATACTGG500.00717130427.999756
TGTTGGT500.00717130427.999758
GTTGGTA500.00717130427.999759
AGACTAA1357.850758E-925.92569469
CTATTAT750.001811731623.3477081
TATGAAA750.00181833923.33312647
GAGACTA1656.603841E-821.21193168
CGAGACT1709.0527465E-820.5880567
GTATATA900.00521630619.4564251
ACGAGAC2005.0048766E-717.49984466
CACGAGA2201.3558183E-615.90894965
CCACGAG2352.6941416E-614.89348464
CCCACGA2701.13321385E-512.96284763
GCCCACG2952.8135282E-511.86430162
AGCCCAC3451.121959E-511.15932161
Produced by FastQC (version 0.11.5)