FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_Gbc_c67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_Gbc_c67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48505
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG150.002212043470.066
GGAGCAG200.00691215852.522
GTACATA200.00691215852.51
CAAGAGA200.00691215852.54
GAATTCG200.00691215852.522
GGCTGTG200.00691215852.540
GTATAGA305.823577E-446.6666641
GTGCTTG450.004268286331.1111091
TCCTTCA450.004268286331.1111094
GTTTCCT500.00714354227.9999981
CCTTCTC750.001807956523.3333341
CGAGCCC1800.0012291213.61111170
CCGAGCC1850.001511292813.24324369
TCCGAGC2050.003267810411.9512268
CTCCGAG2250.00654562510.88888967
Produced by FastQC (version 0.11.5)