FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59949
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG150.00221459570.000018
GCGCTGA150.00221459570.0000118
AGTATAC207.8675206E-570.03
CGGTTTA200.00692007152.521
GTGGACC200.00692007152.528
AAGGGTG200.00692007152.56
GTATACC200.00692007152.54
CAATGTC450.004276249631.11110938
ACAAGCC500.007156786528.00000265
GTATATA1257.669539E-519.61
TTAATTT950.007133077418.42105169
Produced by FastQC (version 0.11.5)