FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70590
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAG150.00221659169.9971622
GCACTCT150.00221659169.9971634
CGCGCAT200.006926264652.49787540
CCCTTGT200.006926264652.49787536
CTGATAG200.006926264652.49787542
CTATAAA450.004282217531.1098521
TGGGCAG450.004282217531.1098525
AATAATC500.007166719527.99886519
CGGACTC657.854337E-426.9410870
GACGGAC800.002632397721.8896370
TTATATA1000.00960540817.499292
TCTCCGA3155.4380755E-511.11854170
ATCTCCG3451.3689858E-410.15171169
CATCTCC3652.4165412E-49.59545368
ACATCTC3854.1269825E-49.09698967
CACATCT4158.78143E-48.433393566
ACACATC4500.00195512977.77746365
TACACAT4950.0049674887.070420764
ATACACA5000.00547718356.999716863
TATACAC5300.0096244946.603506662
Produced by FastQC (version 0.11.5)