FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69042
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGT400.002404370834.9981886
TGCCCAA552.965494E-431.81653410
CCATCTA450.00426662231.13206169
GCCCAAT450.004281805431.109511
ATACTGT450.004281805431.10956
CTGCCCA450.004281805431.10959
ATATGAT500.007166036427.998553
ATAGTAT500.007166036427.998553
CGATATC500.007166036427.9985532
TCAAAGC500.007166036427.9985553
TATACTG755.5153683E-527.9985495
TGTTATC750.001816255223.3321252
GTTATAT800.002642862321.8738671
ATATACT900.00522931219.4434384