FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68469
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT200.006924565452.499452
CAGACCA200.006924565452.499459
ACATTGC400.002403750334.99963431
ATACTAT450.00428070331.1107856
TTGGTTG450.00428070331.1107859
GAATAAA500.007138652328.0201741
TAGAGAG500.007164199427.99970639
TTGCTGT500.007164199427.99970611
CATCTAA500.007164199427.99970635
TGTATAA500.007164199427.9997062
GTATATC657.8495493E-426.9424761
TAGAAGA1000.00960090617.4998174
TCTTATA3950.005310857.974600370
CTCTTAT4050.0066472277.777696669
Produced by FastQC (version 0.11.5)