FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52434
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT150.00221304170.04
ATGTTTC150.00221304170.035
TTATACC200.006915251352.54
TATGCCT400.002398493535.010
TATAAAG400.002398493535.02
ATATTAG400.002398493535.01
GTATATA652.0661879E-532.3076931
AAGCATA500.00714871828.00000211