FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_DGY2071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_DGY2071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48410
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTC150.002212015270.014
AGCCTGT150.002212015270.044
ACTATTC200.00691207252.58
ATCTCAG200.00691207252.57
GGAGAGG200.00691207252.544
TCTCAGC200.00691207252.58
GGCTGCT200.00691207252.518
GTATTAG305.823455E-446.6666641
ATATACT400.002396686535.04
ATAACTA450.004268198731.1111135
GTGTAGA500.007143396428.0000021
TATAAAA755.4798737E-528.02
ATACACA1500.00582750914.069
Produced by FastQC (version 0.11.5)