FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52944
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGA200.00691561652.52
GCTTCGC200.00691561652.550
CCTGAGT200.00691561652.512
TATACAG305.82841E-446.6666645
TGTAGAC350.001244942340.03
GTATAGA552.955292E-431.818181
ATATACA750.001810124623.3333344
TCATATA901.8952519E-423.3333342
TATAATA750.001810124623.3333342
ATAATAT900.00521191419.4444453
Produced by FastQC (version 0.11.5)