FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49005
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTG150.002213074869.992869
TCAGCTA150.002213074869.9928660
TATACCG150.002213074869.992865
ACTCACC200.006915372352.4946448
ACACTGT350.001244666439.995928
GTGTTAT400.002398176434.996431
ATTATAC552.9538918E-431.8149343
ATACCAC450.004270845631.1079373
ATACTAT500.007147812327.9971436
TACAGAA500.007147812327.9971432
TATTATA657.855208E-426.920332
GTATATA952.7310196E-422.1030081
Produced by FastQC (version 0.11.5)