FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58528
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT150.002214335470.08
CTTGCTA150.002214335470.039
GTAGACG150.002214335470.04
CGTCGCT252.3722627E-455.99999634
ATTAGTC200.006919264352.50000411
CTGAACC200.006919264352.50000439
GACGACT200.006919264352.50000449
GCGTCAT200.006919264352.50000458
CTTAACC305.8335136E-446.6666643
TTCGGAT305.8335136E-446.66666450
TATACAG501.6907576E-435.05
TACAGTG450.004275438431.1111097
ATACAGT450.004275438431.1111096
TAATAAT900.005218406719.4444431