FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50117
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATA150.002212471370.02
GAAGCAA200.006913485552.563
ATACTGC200.006913485552.549
TGACCGA200.006913485552.529
CTGCCAA200.006913485552.568
ATAACCG200.006913485552.55
AATAGCC200.006913485552.57
TCTGGTC200.006913485552.540
TAAAGGT400.002397489535.04
ATATTAG450.00426962231.1111091
TATACAC1351.378609E-418.14814870
TTATACA1605.040775E-415.312569
TCTTATA1800.001230116413.61111167
CTCTTAT2000.002717992812.25000166
Produced by FastQC (version 0.11.5)